How can you determine exactley what each of the normal hatchlings of a cross, say the caramel and snow again, would be het for. Is the only sure way to breed back to a parent and see what the outcome is?
There are only 3 possibilities concerning each individual trait in each parent.
1) If the parent IS a mutant, then it carries both copies of the mutant gene and can only pass that mutant gene on to its offspring.
===> All babies will be
at least het for the trait.
2) If the parent is HET for the mutant (heterozygous) carries one normal gene and one mutant gene, so half the time it'll throw the mutant gene and half the time it'll throw the normal gene to its offspring.
===> Half the babies will get the gene from this parent, half won't.
3) If the parent does not carry or express the gene, it can not throw the gene to its offspring.
===> All babies will at MOST be het, never expressing the gene if both parents aren't gene carriers. Babies will not get mutant gene from this parent.
These 3 rules go for each parent.
Lets talk about one gene for now. How about caramel, for grins. Possible crosses involving this gene:
*
Caramel x caramel = 100% Caramels
*
Caramel x het caramel = 50% Caramels, 50% Normals 100% het for caramel
*
Caramel x normal non-het = 100% Normals definitely het caramel
*
Het caramel x het caramel = 25% Caramels, 75% Normals (with 67% chance of being het caramel)
*
Het caramel x normal non-het = 100% Normals (with 50% chance of being het caramel)
*
Normal non-het x normal non het = 100% Normals (non-het)
Each parent either carries 0, 1, or both copies of the gene, so there are only these 6 possibilities for each individual recessive mutation.
If you want KNOWN hets without having to test breed to find the answer, then stick with the crosses producing known hets. As you can see, this pretty much means you have to have one parent that IS the mutant to know for sure. (or that neither parent carries the gene...then you know for sure they aren't het, lol)
Otherwise you are working with percentages.
Het (Aa) x Het (Aa) = 4 possibilities, aa (mutant), aA and Aa (hets), and AA (non-het).
===> 1/4 (25%) mutants, 3/4 (75%) normals,
of those 3 normals, 2 should be het, so the normals have a 2/3 chance of being het = 67%.
Het (Aa) x Non-het (AA) = AA, AA, aA, aA
===> 2/4 (50%) Non-hets, 2/4 (50%) Hets. All appear normal, so each has a 50% chance of being het for the trait and the only way to know is to test cross them to a known carrier or mutant.
In your example of snow x caramel, you are working with 3 traits (amel, anery, caramel).
If you breed the snow (amel + anery) to a caramel, all offspring should be normal (if neither parents carry any hets) and all will definitely be het for amel and anery (from the snow) and caramel (from the caramel).
Breeding your normals het for amel, anery, and caramel together would be expected to give you:
27/64 Normals (67% het amel, anery, and/or caramel)
9/64 Amels (67% het anery and/or caramel)
9/64 Anerys (67% het amel and/or caramel)
9/64 Caramels (67% het amel and/or anery)
3/64 Snows (67% het caramel)
3/64 Butters (67% het anery)
and the monkey wrench:
3/64 Anery caramels (67% het amel) == anery masks caramels, so these are expected to look just like anerys even though they are also caramels.
1/64 Butter anerys == again, anery masks caramel, so they should look like regular snows, more or less. Undistinguishable from regular snows.
Because of masking, you would expect:
27/64 Normals
9/64 Amels
12/64 Anerys (25% homozygous for caramel/50% het caramel)
(i.e. 75% chance that it AT LEAST carries caramel)
4/64 Snows (25% homo caramel/50% het caramel)
3/64 Butters
in the second generation crossing your F1 hets for snow and caramel.
FYI here's a link where this cross was discussed:
Discussion of anery and caramel masking
***Disclaimer: Didn't have time to proof this, I'll check it over tonight.