I'm a certified veterinary technician and did this in school after learning the laws of humane euthanasia. I haven't watched the video yet, so I'll just tell you what I remember. It's required by law (in the veterinary industry at least) to have the rodent unconscious (we used anesthesia, but you can probably just stun them). Then use a pen or screwdriver like Buzz said and yank the tail hard. I was a little squeamish when I did it and didn't yank hard enough the first time and had to do it again. That's why they should be unconscious. There's a gross popping noise which is the dislocation. As stated, muscle twitching is normal after death, it's just electrical activity. Check for a heart rate by feeling the chest. If you're not sure, you can always pull again. We didn't do it on rats. I would just be careful because there's something called "tail slip" that can happen when holding them by the tail where the skin can slip off the tail. You can probably google humane euthanasia techniques for laboratory rodents and find some of the guidelines required and find some step by step instructions. I'd be more comfortable knowing that there's been research with the technique and you know it's humane.